NASC Stock Detail Page
NASC ID: N68978
Name: vip4-1
ABRC stock number: CS68978
Description: Insertional mutagen into introgression line Col:FRI (obtained from Rick Amasino) using vector pPZP201 modified to contain a gene for resistance to glufosinate. Insertion is within 10th (of 11) exon. Mutant was isolated from a population of T-DNA-mutagenized plants in a screen for plants with a cold-independent, early flowering phenotype. Mutant was backcrossed 5 times to Col-0 with final selection by progeny testing. Mutant retains resistance to glufosinate.
Donation Date: 2015-03-27
| Donated by: | Michigan State University Steve van Nocker |
Donor Number: vip4-1
Stock type: individual line
Material type: seed
Comment: Donors: Steve Van Nocker.Clone name: pPZP201:BAR. Parents: Col(Columbia). In publications involving this stock, please acknowledge the donor and ABRC. Example template: vip4-1 was a gift from Steve van Nocker, and is made available through the Arabidopsis Biological Resource Center (CS68978). heterozygous.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Background: Col(Columbia)
Pedigree: Mutant was backcrossed 5 times to Col-0 with final selection by progeny testing.
Growth requirement: Homozygotes have reduced sterility at typical growth temperatures (22-24C) and above.
Phenotype
Phenotypes for homozygous vip4-1 mutants after 2-5 day stratification are the following: slightly early flowering due to repression of FLC; slight floral abnormality with sepals fringed with white and failing to enclose floral bud; variable fertility under standard growth conditions. Phenotypes are increasingly severe when plants are grown at higher temperatures (above 22 C).
References
Zhang, H. & van Nocker, S. 2002. The VERNALIZATION INDEPENDENCE 4 gene encodes a novel regulator of FLOWERING LOCUS C. The Plant Journal 31(5):663-73.PubMed ID: 12207655.